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Acute exposure to homocysteine (50 μM–1 mM for 24 h) led to activation of the Nrf2 pathway (with a peak expression of Nrf2 3 h after Hcy administration), increased expression of genes encoding antioxidant enzymes, including NQO1, CAT, SOD2, HMOX1, and GPX1, and a concomitant decrease in ROS and alleviation of oxidative stress [56]