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The dose of methotrexate was based on literature showing neurotoxicity at 1 μM ( FIGURE 1 2.4 Tissue processing and immunohistology At experimental endpoints, brain organoids were placed directly in 16% methanol-free formaldehyde (Polysciences) diluted in DPBS (Gibco) overnight at 4°C on a rocking shaker (see Supplementary Table 1 for catalog numbers for all reagents)
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