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To avoid confusion, cellular metal availabilities have been expressed as free energy changes, Δ G , associated with in vivo metal complex formation: the values for metal concentrations can be used to calculate the Δ G using the standard equation RTln K A , where K A is the affinity of a molecule that would be 50% saturated with metal at the available buffered concentration [93]
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The plasma and extracellular fluid include transport proteins that bind cobalamin: about 75% of total plasma cobalamin in dogs and cats is associated with transcobalamin II
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