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For the reaction, 2 µg of the total RNA were combined with water pretreated with diethylpyrocarbonate (DEPC-H 2 O, Thermo Fisher Scientific, Waltham, MA, USA) and with 1 µL of Oligo(dT) 15 primer (0.5 µg/reaction, Thermo Fisher Scientific, Waltham, MA, USA) and incubated for 5 min at 70 °C
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