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The next morning, the overnight culture was subsequently diluted 100:1 in fresh LB medium supplemented with the same antibiotics and incubated for 2–3 h until reaching an optical density at 600 nm (OD 600 = 0.4–0.5)
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[DOI] [PubMed] [Google Scholar] 114.Benhar M, Forrester MT, Stamler JS
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