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Williams argued that the electrode potential of the cytosol favours iron(II) over iron(III) and because the iron(II) binding constants for many cytoplasmic enzymes fall in the range 10 −8 –10 −7 M [5,7], they require a similar standing concentration of iron(II) in order to prevent dissociation from the enzyme

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Therefore, the efficiency of analyte deprotonation is often low, consistent with the typically low-intensity anion analyte signals obtained using acidic matrices