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The desalted sample was evaporated to dryness in a speedvac and then resuspended in 10 μL Buffer A and analyzed by mass-spectrometry using the following LC-MS gradient: 5% buffer B in buffer A from 0-15 min, 5–15% buffer B from 15–17.5 min, 15–35% buffer B from 17.5–92.5 min, 35–95% buffer B from 92.5–95 min, 95% buffer B from 95–105 min, 95–5% buffer B from 105–107 min, and 5% buffer B from 107–125 min

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Therefore, such shuttle mechanisms enable cells to maintain adequate NAD + in the cytosol in support of glycolysis and sufficient NADH in the mitochondria in support of OXPHOS