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A two-hour gap between NMN and the injection is sufficient to minimize any combined GI effects
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Expression was confirmed with an anti-FLAG antibody in western blot where the fusion protein showed an approximate size of ∼71 kDa which is in agreement with the expected size (MSMEG_0311 ∼ 43 kDa, GFP ∼27 kDa and FLAG tag ∼ 1 kDa) (Fig
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