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Thus, participants in whom chelation would potentially have been indicated on the basis of higher body burdens of toxic elements known to be associated with cardiovascular disease were not identified, and it is unknown if additional benefit may have accrued with additional treatment, among those with remaining significant body burdens of heavy metals

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4-Morpholineethanesulfonic acid hydrate) was mixed with 10 µM DTT (BioShop) and incubated for 10 min at 55 °C