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There was evidence that mice without efficient expression of SOD2 enzyme resulted in “significant epidermal thinning.” In humans “mitochondrial dysfunction” promotes “cellular senescence in epidermal keratinocytes in culture.” [7] This means because of senescence, there were fewer cells in the epidermis, causing it to be thinner

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Reagents The following antibodies were used: β-actin/ACTB (Abcam, Cambridge, UK, #6276), GAPDH (Abcam, #8245), MAP1LC3B/LC3B (Cell Signaling, Beverly, MA, USA, #3868), HMOX1 (Enzo, Farmingdale, NY, USA, #ADI-OSA-110), IgG Fcγ fragment specific (Jackson ImmunoResearch Laboratories, West Grove, PA, USA, #109-006-098), FK2 antibody recognizing mono- and polyubiquitinated proteins (Enzo, #BML-PW-8810), SQSTM1 (Progen, Heidelberg, Germany, #GP62-C), SRXN1 (Proteintech, Chicago, IL, USA, #14273-1-AP), xCT (Cell Signaling, Beverly, MA, USA, #12691)