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Preparative cultures (1 l) for each transformed strain were incubated with shaking at 37°C in lysogeny broth (LB) supplemented with 5 mM AcK (A4021-5G Sigma), 10 µM sodium selenite (10102-18-8, AlfaAesar), and appropriate antibiotics (100 μg/ ml ampicillin (BP1760-25, Fisher) for pET and 34 μg/ ml chloramphenicol (02930-100G, Ampresco) for pTech)

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[6] Levels in the borderline range warrant careful clinical assessment alongside symptoms