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The foundational study (Neelakantan et al., Biochemical Pharmacology, 2018) dosed diet-induced obese mice with 5-Amino-1MQ at 20mg/kg, three times daily subcutaneously, for 11 days, and reported reduced body weight and white adipose mass, smaller adipocytes, and lower plasma cholesterol — notably, without any change in food intake, distinguishing its mechanism from appetite-suppressant compounds

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[9] High-dose niacin (used to treat cholesterol) lacks human safety data in pregnancy and could theoretically affect the fetus (for example, by causing hypotension or altering lipid metabolism)